Rapid test for determination of esculin hydrolysis.
نویسندگان
چکیده
An esculin hydrolysis test is described which distinguishes over 97% of bacteria that can convert esculin to esculetin in a buffered solution, from those that cannot, within an hour.
منابع مشابه
Rapid spot test for the determination of esculin hydrolysis.
Esculin hydrolysis is a useful test in the differentiation of both gram-positive and gram-negative bacteria covering a wide spectrum of aerobes, facultative anaerobes, and anaerobes. Commonly utilized methods require a minimum of 18 h of incubation in broth or agar medium and utilize the production of a brown-black compound, due to the combination of ferric ions with the hydrolysis product escu...
متن کاملComparison of spot esculin hydrolysis with the PathoTec strip test for rapid differentiation of anaerobic bacteria.
The ability of several anaerobic bacteria to hydrolyze esculin to esculetin is used by clinical microbiologists and taxonomists in the differentiation and identification of both gram-positive and gram-negative microorganisms. Conventional methods used for determining esculin hydrolysis by anaerobic bacteria require 24 to 48 h for completion. In this paper we evaluate two procedures which yield ...
متن کاملEsculin hydrolysis reaction by Escherichia coli.
The literature contains variable reports concerning the hydrolysis of esculin by members of the family Enterobacteriaceae and particularly Escherichia coli. We examined 113 strains of fresh clinical isolates of E. coli and assessed the ability of colonies in a population to hydrolyze esculin with and without preincubation in inducible substrates at 24, 48, and 72 h. The number of strains capabl...
متن کاملEsculin hydrolysis by Enterobacteriaceae.
Literature reports disagree concerning esculin hydrolysis in the family Enterobacteriaceae. A total of 2,490 strains of the family were investigated for esculin hydrolysis by two methods, the esculin spot test and the PathoTec incubation strip, which measures constitutive enzyme, and five growth-supporting methods, which determine both constitutive and inducible enzymes. The five growth-support...
متن کاملSodium chloride-esculin hydrolysis test for rapid identification of enterococci.
The ability of enterococci to cause severe disease in humans and their relative resistance to chemotherapeutic agents make it desirable to rapidly differentiate these organisms from other streptococci. We developed and evaluated a test that within 2 h distinguishes enterococci from other alpha-, beta-, or nonhemolytic streptococci in a buffered solution containing 0.2% esculin and 5% sodium chl...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 12 3 شماره
صفحات -
تاریخ انتشار 1980